measure respiration at the micro scales
The Unisense MicroRespiration system enables researchers to measure O2, H2, H2S, N2O, NO, pH, Redox and temperature at micro scales in sealed systems.
The MicroRespiration System is an excellent tool to measure minute analyte changes in closed chambers. This could be respiration rates of phyloplankton, H2S production in macrophages or pH changes in bacterial suspensions. The two central features of the MicroRespiration System are gas tight glass chambers and protected microsensor.
- Measurement of two analytes simultaneously
- Oxygen MicroOptode sensor with automatic temperature compensation
- View metabolic rates online and in real time
- Sealed chambers in pressure equilibrium with environment
- All materials in contact with sample are autoclavable
- Protected stirring of fragile samples
- Excellent for teaching purposes
The glass chambers are designed to be completely sealed when immersed in water or sealed with oil. Samples are placed in the chambers and the analyte concentrations are measured through a thin capillary in the chamber lids with the MicroRespiration microsensor. The chambers are made in volumes from 400 µL to 400 mL. In addition to this you can have double chambers for measurement of two analytes simultaneously and injection lids to inject substrates to the samples while measuring.
The Unisense MicroRespiration microsensors have insignificant analyte consumption and a very low detection limit that allows for detection of minute concentration changes in the chambers. Correct insertion of the microsensor through the capillary is secured by the aluminum guide protecting the microsensor.
Below is a list of Unisense Microsensors suitable for the MicroRespiration System:
- O2 Microsensor
- O2 Micro-Optode Sensor
- H2S Microsensor
- H2 Microsensor
- N2O Microsensor
- NO Microsensor
- Temperature Sensor
- Redox Microelectrode
- pH Microelectrode
The aluminum guide fits perfectly into the MicroRespiration Rack, which holds up to eight chambers (< 4mL). The MicroRespiration Rack guides the microsensor directly into the chambers making the MicroRespiration System easy to use and excellent for teaching purposes. The MicroRespiration Rack has separate stirring of the chambers ensuring homogeneity in the samples. Even fragile samples can be stirred due to a metal net separating the sample from the magnet.
SensorTrace Rate Software
The matching software SensorTrace Rate provides real-time and online PC inspection of data and enables measurement and calculation of analyte respiration and metabolism rates in several samples simultaneously. This software allow you to measure and analyze multiple analytes and multiple samples.
Below is a list of Unisense Multimeters, Monometers and Amplifiers:
Alonzo,F. et al (2008), Increased effects of internal alpha irradiation in Daphnia magna after chronic exposure over three successive generations, Aquatic Toxicology, 146 – 156, vol. 87
Brodersen,K.P. et al (2007), Respiration of midges (Diptera; Chironomidae) in British Columbian lakes: oxy-regulation, temperature and their role as palaeo-indicators, Freshwater Biology, 593 – 602, vol. 53
Grasselli,E. et al (2011), Non-receptor mediated actions are responsible for the lipid-lowering effects of iodothyronines in FaO rat hepatoma cells, Journal of Endocrinology, JOE – 11, vol.
Sommer,S. et al (2009), Benthic respiration in a seep habitat dominated by dense beds of ampharetid polychaetes at the Hikurangi Margin (New Zealand), Marine Geology, – , vol.
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