With the NanoRespiration System you can measure respiration rates on single individuals such as copepod eggs, phytoplankton or zebrafish eggs. The system has high accuracy and respiration rates down to 0.7 fmol/s can be determined.
- Determine extremely low oxygen consumption rates
- Fast and repetitive measurements of several samples
- Accurate and non-destructive measurements by Unisense O2 microsensor
- Autoclavable capillaries
The NanoRespiration System requires exact positioning and moving of the microsensor tip. This is ensured by integration of the NanoRespiration System into the Unisense MicroProfiling System. Easy operation of the The MicroProfiling System and data logging is done with SensorTrace Profiling and oxygen respiration rates are calculated using a free spread sheet.
Samples are placed in oxygen impermeable and chemical inert glass capillaries with sealed bottoms. Each sample is overlaid with appropriate medium and when the setup has reached steady state an oxygen gradient will build up in the thin capillary. This gradient is measured by a very fine-tipped Unisense microsensor, and the respiration rate of the microorganism placed in the capillary can be calculated from the oxygen gradient.
The NanoRespiration System was developed for determining oxygen respiration rates; however the system is compatible with all Unisense microsensors. In 2006 Riisgaard-Pedersen published a study in Nature in which the NanoRespiration System was used for measuring denitrification by Nonionella cf. stella. based on N2O accumulation in the NanoRespiration Capillaries.
oxygen sensor, hardware and software
The Unisense NanoRespiration System utilises the oxygen microsensor – a Clark-type amperometric sensor. These unique oxygen sensors can measure dissolved or atmospheric oxygen concentration through a 10μm diameter tip. The sensors minimally disturb the sample and can be used repeatedly across a number of experiments.
The oxygen microsensor is supported by the Unisense multi-channel picoammeter or OXY-Meter. All data is collected with the SensorTrace software and respiration rates are calculated with the SensorTrace Respiration module.
Nielsen,P. et al (2007), Respiration rates of subitaneous eggs from a marine calanoid copepod: monitored by nanorespirometry, Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, 287 – 296, vol. 177. Link
Lopes,A.S. et al (2005), Respiration rates of individual bovine in vitro-produced embryos measured with a novel, non-invasive and highly sensitive microsensor system, Reproduction, 669 – 679, vol. 130. Link
Abe,H. (2007), A Non-invasive and Sensitive Method for Measuring Cellular Respiration with a Scanning Electrochemical Microscopy to Evaluate Embryo Quality, Journal of Mammalian Ova Research, 70 – 78, vol. 24. Link