measure dissolved N2O gas

Unisense N2O-25The Unisense N2O microsensor is a unique sensor offering the possibility of measuring dissolved and gaseous N2O. With a detection limit of less than 0,5 µM and tip sizes down to 25 µm, the Unisense N2O microsensor is a high quality research tool for reliable and fast measurements.

The microsensor construction makes it possible to use the N2O microsensor in a broad variety of research fields where non-destructive, fast and accurate measurements are required. The N2O microsensor is designed for research applications within:

  • Wastewater research
  • Environmental sciences
  • Biomedical sciences
  • Biotechnology

The N2O-R sensor is a more robust version of the normal nitrous oxide sensor with optimized sensitivity and protection cap suited for bulk measurements including wastewater applications.

Download Unisense Microsensors Brochure

microsensor technology

Unisense N2O-RThe Unisense nitrous oxide microsensor is a miniaturized Clark-type sensor with an internal reference and a guard cathode.

In addition, the sensor is equipped with an oxygen front guard, which prevents oxygen from interfering with the nitrous oxide measurements.

The sensor is connected to a high-sensitivity picoammeter and the cathode is polarized against the internal reference. Driven by the external partial pressure, nitrous oxide from the environment will penetrate through the sensor tip membrane and be reduced at the metal cathode surface. The picoammeter converts the resulting current to a signal. The internal guard cathode is also polarized and scavenges N2O in the electrolyte, thus minimizing zero-current and polarization time.

For special applications, some versions of the N2O microsensor can be customized to have extremely high sensitivity (25 nM). Please view specifications.


part numbers and outside tip diameter

  • PageLines-UnisenseMultimeter-FRONT.jpgN2O-25: 20-30 µm
  • N2O-50: 40-60 µm
  • N2O-100: 90-110 µm
  • N2O-500: 400-600 µm
  • N2O-R: Mounted cap
  • N2O-MR: 400-600 µm
  • N2O-N: 1.6 x 40 mm
  • N2O-NP: 1.6 x 40 mm – needle sensor for piercing



Download Detailed N2O Microsensor Spec Sheet



Download N2O Sensor Manual


Biofilm Microprofilingapplications




Andersen,K. et al (2001), An oxygen insensitive microsensor for nitrous oxide, Sensors and Actuators B-Chemical, 42 – 48, vol. 81

Elberling,B. et al (2010), High nitrous oxide production from thawing permafrost, Nature Geoscience, 332 – 335, vol. 3

Horn,M. et al (2003), The Earthworm Gut: an Ideal Habitat for Ingested N20-Producing Microorganisms, Applied and Environmental microbiology, 1662 – 1669, vol. 69

Kampschreur,M.J. et al (2008), Dynamics of nitric oxide and nitrous oxide emission during full-scale reject water treatment, Water Research, 812 – 826, vol. 42

Liengaard L, et al (2013) Extreme emission of N2O from tropical wetland soil (Pantanal, South America). Frontiers in Microbiology.  3:433. doi: 10.3389/fmicb.2012.00433

Zhou,Y. et al (2008), Development of a 2-sludge, 3-stage system for nitrogen and phosphorous removal from nutrient-rich wastewater using granular sludge and biofilms, Water Research, 3207 – 3217, vol. 42


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