dissolved nitric oxide measurements
Nitric oxide plays an important role in many biological processes; however, natural concentrations are often in the nanomolar range. The Unisense NO microsensor is characterized by a very high sensitivity, which enables you to measure minute concentrations of NO. Combined with a fast response time the Unisense NO microsensor is an excellent research tool for a broad range of tasks.
The sensor can be used equally well for measurements in tissue, air, or liquid, and is applied in a broad variety of research fields where non-destructive, fast and accurate measurements are required.
The Unisense nitric oxide microsensor has been used by the world’s leading scientists for decades and is cited in over hundreds of papers in journals such as Nature and Science.
- Measurement tip size as small as 2 µm
- Extremely fast T90 response times of less than 0.3 seconds
- Suitable for a wide range of applications from biogeochemistry to biomedical
The NO micro- and minisensors are Clark-type sensors measuring the external NO partial pressure. The working principle of the sensor is based on diffusion of NO through a silicone membrane to an NO oxidizing anode, which is polarized against an internal counter cathode. The resulting sensor signal is in the pA range and is measured by a high quality picoammeter e.g. the Unisense Microsensor Multimeter.
Compared to other standard NO sensors the Unisense NO microsensor has an extremely low consumption of max. 935 pmol/hour corresponding to 0,1 %/hour in a 1 mL 0,1uM NO sample.
tip diameter sizes
- NO-10: 8-12 µm
- NO-25: 20-30 µm
- NO-100: 90-110 µm
- NO-500: 400-600 µm
- NO-MR: 400-600 µm
- NO-NP: 1.6 x 40 mm – needle sensor for piercing
- Biogeochemistry and ecosystem functioning
- In-vitro cell culture profiling
- Biomedical biofilm research
- Micro-volume measurements
Schreiber,F. et al (2008), Nitric Oxide Microsensor for High Spatial Resolution Measurements in Biofilms and Sediments, Anal.Chem, 1152 – 1158, vol. 80
Aamand,R. et al (2009), Generation of nitric oxide from nitrite by carbonic anhydrase: a possible link between metabolic activity and vasodilation, American Journal of Physiology- Heart and Circulatory Physiology, H2068 – , vol. 297
Ettwig,K.F. et al (2010), Nitrite-driven anaerobic methane oxidation by oxygenic bacteria, Nature, 543 – 548, vol. 464
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